1. The shape of RNA polymerase is compared with
1) Claw of bird 2) Hand of man 3) Claw of Tiger *4) Claw of Crab
2. The part of RNA polymerase that shows promoter specificity is
1) α unit 2) β unit 3) β′ unit *4) σ unit
3. The activator of RNA polymerase is
1) K+ *2) Mg+2 3) Mn+2 4) Zn+2
4. [A]: Core enzyme of RNA polymerase can bind to any part of DNA.
[R]: The ability of RNA polymerase to bind to any non-specific sequences of DNA molecule is reduced by σ factor.
1) Both A and R are true and R is the correct explanation of A
*2) Both A and R are true but R is not the correct explanation of A
3) A is true but R is false 4) A is false but R is true
5. The part of RNA polymerase that does not remain with the enzyme throughout transcription but remains only upto transcription of < 10 bases is
*1) σ 2) β 3) β′ 4) α
6. Promoter sequence of DNA is read by amino acids of
I. σ unit II. α-unit III. Β unit IV. 
unit
unit1) I and III 2) II and IV *3) I and II 4) II and III
7. The consensus sequence of promoter region is
1) Tetramer 2) Octamer *3) Hexamers 4) Decamers
8. The length of transcription bubble is
*1) 12 – 14 bp 2) 8 – 10 bp 3) 6 – 8 bp 4) 16 – 20 bp
9. [A]: Coding strand is called as non-template strand.
[R]: Coding stand is not involved in transcription.
*1) Both A and R are true and R is the correct explanation of A
2) Both A and R are true but R is not the correct explanation of A
3) A is true but R is false 4) A is false but R is true
10. The length of RNA-DNA hybrid during transcription is
*1) 8-9 bp 2) 6-7 bp 3) 10 -11 bp 4) as long as mRNA
11. During elongation of transcription, the following part of RNA polymerase is not present.
*1) σ 2) 
3) β 4) α
3) β 4) α12. ‘DNA melting’ means
1) Degradation of non-template strand 2) Degradation of both strands of DNA
*3) Unwinding of DNA due to breakage of ‘H’ Bonds 4) Cutting the DNA into fragments
13. Termination of transcription requires
1) G-C rich hair pin in coding strand 2) G-C rich hair pin in template strand
*3) G-C rich hairpin in mRNA 4) A-U rich hair pin in mRNA
14. The region of mRNA where Rho factor is attached is called as
*1) rut 2) rat 3) ret 4) rit
15. In Eukaryotes hnRNA synthesis is catalyzed by
1) RNA Polymerase I 2) RNA polymerase III *3) RNA polymerase II 4) RNA polymerase IV
16. rRNA is mainly formed in
1) Nucleoplasm 2) Cytoplasm 3) Nuclear envelope *4) Nucleolus
17. [A]: RNA polymerase can recognize the promoter region.
[R]: DNA polymerase can initiate DNA synthesis.
1) Both A and R are true and R is the correct explanation of A
2) Both A and R are true but R is not the correct explanation of A
*3) A is true but R is false 4) A is false but R is true
18. Messenger RNA accounts for ----- % of total cellular RNA
1) 10-15 *2) 2-5% 3) 5 – 10% 4) 1-2%
19. 5S ribosomal RNA of Eukaryotes is synthesized by
1) Any RNA polymerase 2) RNA polymerase I 3) RNA polymerase II *4) RNA polymerase III
20. Choose the correct statement with respect to translation.
1) Aminoacy tRNA is shifted from A site to P site of Ribosme
*2) Peptidyl tRNA is shifted from A site to P site of Ribosme
3) Deacylation of Peptidyl tRNA takes place at E site 4) tRNA is acylated at A site
21. The following translation factor is also called as anti-association factor
*1) IF3 2) IF2 3) IF1 4) EFG
22. This is Shine-Dalgarno sequence
*1) AGGAGG 2)ACCACC 3) UGGUGG 4) CGGCGG
23. Initiater tRNA is indicated as
*1) t-RNAf 2) tRNAm 3) tRNAi 4) tRNAf
24. The factor that helps in loading the initiator tRNA at P site is
1) IF3 *2) IF2 3) IF1 4) EFTS
25. Multiple functions are executed by the following translation factor.
1) EF3 *2) IF3 3) RF3 4) IF2
26. [A]: Terminator is the sequence present on DNA template
[R]: It terminates the synthesis DNA
1) Both A and R are true and R is the correct explanation of A
2) Both A and R are true but R is not the correct explanation of A
3) A is true but R is false 4) A is false but R is true
27. The nitrogen base present at 5′ end of initiator tRNA is
*1) Cytosine 2) Guanine 3) Adenine 4) Uracil
28. The factor responsible for loading aminoacy tRNA at A site is
1) EFG 2) EFTS *3) IFTU 4) EFP
29. The translation that binds to neither larger subunit nor smaller subunit of ribosome is
1) EFTU *2) EFTS 3) EFG 4) IF3
30. [A]: During chain elongation GDP is displaced by EFTS.
[R]: GTP displaces EFTs from EFTU
1) Both A and R are true and R is the correct explanation of A
*2) Both A and R are true but R is not the correct explanation of A
3) A is true but R is false 4) A is false but R is true
31. The requirements for binding the aminoacyl tRNA at A site is/are
I. GTP II. EFTs III. EFTU IV. GDP
*1) I and II 2) II and III 3) III and IV 4) I and III
32. The antibiotic that inhibits the function of EFTU is
1) Kanamycin *2) Kirromycin 3) Puromycin 4) Streptomycin
33. [A]: E site is exclusively present on larger subunit
[R]: P site is exclusively present on smaller subunit
1) Both A and R are true and R is the correct explanation of A
2) Both A and R are true but R is not the correct explanation of A
*3) A is true but R is false 4) A is false but R is true
34. During translocation
1) Both subunits of ribosome move at a time towards 3′ end of mRNA
*2) First there is shift of 50S subunit towards 3′ end and later 30S subunit
3) First there is shift of 30S subunit towards 3′ end and later 50S subunit
4) First there is shift of deacylated tRNA to E site and later ribosomal shift.
35. Fusidic acid prevents the release of following factor from ribosome
*1) EFG 2) EFTU 3) EFTS 4) RF336. 36. [A]: Puromycin inhibits peptide bond formation
[R]: In resembles aminoacid attached to terminal adenosine of tRNA
*1) Both A and R are true and R is the correct explanation of A
2) Both A and R are true but R is not the correct explanation of A
3) A is true but R is false 4) A is false but R is true
